Jablonski Diagram
Laser
400
500
600
700
800
900
1000
1097
nm
Higher energy and vibration states
Lowest singlet excited state
Excitation
599 nm
Emission
674 nm
Ground state
Single Photon Excitation
Delay: 10-20 sec
4
3
2
1
0
2
1
0
Incident
Photon
Excitation
10-20 s
Relaxation
10-20 s
Fluorescence
10-20 s
Excitation Delay
Start
«Drag