Solvent Effects on Fluorescence Emission
S1
Jablonski
Energy
Diagram
Ground State (S0)
Solvent
Solvent
Solvent
Relaxed
S1
Non-Polar
Solvent Not
Affected by
Fluorophore
Dipole
Fluorophore
100
80
60
40
20
0
Relative Intensity
300
400
500
600
700
Wavelength (Nanometers)
Absorption
Emission
Start